DcR3 suppresses LPS-Induced Aggresome-like induced structures in Macrophages via inhibition of ROS and p38 MAPK
نویسندگان
چکیده
Abstract Decoy receptor 3 (DcR3), also known as tumor necrosis factor (TNFR) superfamily member 6b (TNFRSF6B), is a pleiotropic which modulates cell functions via decoy and non-decoy actions. DcR3 has been shown its anti-apoptotic anti-inflammatory effects on cancers inflammatory diseases. Toll-like receptors (TLRs), are the members of pattern recognition (PRRs), trigger response against invading pathogens. However, it remains unclear whether plays role in TLR4-induced response. In this study, we demonstrated that bone marrow-derived macrophages (BMDMs) did not affect lipopolysaccharide (LPS)-induced COX-2, iNOS, NLRP3 pro-IL-1b protein expression. It reported aggresome-like induced structures (ALIS) formed to LPS macrophages. ALIS, containing ubiquitinated aggregates, stress-induced involved MHC class I antigen presentation. Our findings illustrated can decrease LPS-induced ALIS formation inhibiting cellular ROS production p38 MAPK phosphorylation. addition LPS, ZnPP-, bafilomycin A1- MG132-induced expression were reduced by DcR3. Confirming notion controlled autophagy, found rapamycin formation, while A1 increase LC3-II accumulation with similar extent WT Nevertheless, does change p62 HO-1 LPS. Taken together, despite fact alter response, our data suggest novel suppression formation. MOST 111-2320-B-002-028
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ژورنال
عنوان ژورنال: Journal of Immunology
سال: 2023
ISSN: ['1550-6606', '0022-1767']
DOI: https://doi.org/10.4049/jimmunol.210.supp.168.02